F1763
Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
FITC conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Conditions de stockage
protect from light
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
indirect immunofluorescence: 1:320
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.
Spécificité
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.
Immunogène
Purified rat IgG
Application
Anti-Rat IgG (whole molecule)-FITC antibody has been used:
- in immunolabelling for confocal laser scanning microscopy (CLSM)
- in immunostaining
Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.
Actions biochimiques/physiologiques
IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
Autres remarques
Antibody adsorbed with human IgG.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Notes préparatoires
Adsorbed to reduce background staining with human samples.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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