D8156

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

• Synonyme(s) : Monoclonal Anti-Digoxin
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: DI-22, monoclonal
• Contient: 15 mM sodium azide
• Technique(s): dot blot: suitable; flow cytometry: suitable; indirect ELISA: 1:10,000 using digoxin-BSA; indirect ELISA: 1:2,500 using digoxigenin-transferrin
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Spécificité

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.

Immunogène

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• dot blot
• flow cytometry
• fluorescence in situ hybridization (FISH)
• DNA hybridization
• in-situ hybridization (ISH)
• immunodetection of the probe and chromosome X

Actions biochimiques/physiologiques

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.

Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable