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D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

Synonyme(s) :

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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About This Item

Numéro CAS:
9003-98-9
Numéro de classification (Commission des enzymes):
3.1.21.1 (BRENDA, IUBMB)
Numéro CE :
232-667-0
Numéro MDL:
MFCD00130918
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.53

Source biologique

bovine pancreas

Niveau de qualité

200

Qualité

for molecular biology

Forme

buffered aqueous glycerol solution

Activité spécifique

10000 units/mg protein





Poids mol.

29.1 kDa

Concentration

5—15 mg protein/mL

Technique(s)

DNA extraction: suitable

Adéquation

suitable for molecular biology

Numéro d'accès UniProt

P00639

Application(s)

cell analysis

Activité étrangère

RNase, none detected

Température de stockage

−20°C

Informations sur le gène

cow ... DNASE1(282217)

Description générale

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Composants

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Définition de l'unité

One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Autres remarques

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Inhibiteur

Réf. du produit
Description
Tarif

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution

R0884

T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution

D2886

DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution

P4978

Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution

P4390

Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution

G5663

Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution

R6513

Ribonucléase A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonucléase A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

P6911

Protéase from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

AMPD1

DNase I, Amplification Grade

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
Umesh Kumar Dhawan et al.
Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)
Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
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Protocoles

Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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