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D4418

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonyme(s) :

DAB, DAB tablets, Immunohistology substrate

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About This Item

Numéro CAS:
91-95-2
Numéro MDL:
MFCD00007725
Code UNSPSC :
12352204
ID de substance PubChem :
24893872
Nomenclature NACRES :
NA.83

product name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 15 mL

Niveau de qualité

200

Forme

tablet

Solubilité

water: 1 tablet/5 mL

Température de stockage

−20°C

Chaîne SMILES 

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

Clé InChI

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Catégories apparentées

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Informations légales

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Erin N Scully et al.
Behavioural brain research, 331, 151-158 (2017-05-17)
Zebra finches (Taeniopygia guttata) are sexually dimorphic songbirds, not only in appearance but also in vocal production: while males produce both calls and songs, females only produce calls. This dimorphism provides a means to contrast the auditory perception of vocalizations
Huiming Yang et al.
Nature communications, 11(1), 2582-2582 (2020-05-24)
Polyglutamine expansion in proteins can cause selective neurodegeneration, although the mechanisms are not fully understood. In Huntington's disease (HD), proteolytic processing generates toxic N-terminal huntingtin (HTT) fragments that preferentially kill striatal neurons. Here, using CRISPR/Cas9 to truncate full-length mutant HTT
F Gualtieri et al.
Scientific reports, 9(1), 7129-7129 (2019-05-11)
In the mammalian brain, adult hippocampal neurogenesis (AHN) is suppressed by chronic stress, primarily at the ventral pole of the hippocampus. Based upon anatomy, we hypothesise that the caudal pole of the avian Hippocampal Formation (HF) presents a homologous subregion.
Monika Verma et al.
Toxicon : official journal of the International Society on Toxinology, 139, 58-65 (2017-09-19)
Anthrax associated causalities in humans and animals are implicated mainly due to the action of two exotoxins that are secreted by the bacterium Bacillus antharcis during the infection. These exotoxins comprise of three protein components namely protective antigen (PA), lethal
Ya-Chao Wang et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 980-993 (2016-05-14)
The NMDA antagonist memantine preferentially inhibits extrasynaptic NMDA receptors, which are overactivated upon stroke and thought to disturb neuroplasticity. We hypothesized that memantine enhances post-ischemic neurological recovery, brain remodeling, and plasticity. C57BL6/j mice were exposed to intraluminal middle cerebral artery
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Protocoles

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Lyse cellulaire et extraction protéique pour le western blotting

Protocole de préparation d'échantillons pour la lyse cellulaire et une bonne extraction des protéines à partir de cellules et de tissus en culture en vue d'un western blotting ultérieur.

Lyse cellulaire et extraction protéique pour le western blotting

Protocole de préparation d'échantillons pour la lyse cellulaire et une bonne extraction des protéines à partir de cellules et de tissus en culture en vue d'un western blotting ultérieur.

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