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Principaux documents

D3159

Sigma-Aldrich

Deoxyribonucleic acid from herring sperm

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp), degraded

Synonyme(s) :

Deoxyribonucleic acid, partially degraded from herring sperm, hsDNA

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About This Item

Numéro CE :
309-566-6
Numéro MDL:
MFCD00130921
Code UNSPSC :
41106305
eCl@ss :
32160414
Nomenclature NACRES :
NA.51
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Forme

powder

Adéquation

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp)

Température de stockage

2-8°C

Description générale

Herring Sperm DNA, also known as hsDNA is a natural source of DNA designed for DNA binding agent studies. (1) The detection limits of hsDNA are around 5.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-2.5 mµg.[1]

Application

Herring sperm DNA (hsDNA) is a natural DNA used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Herring sperm DNA is also used in physicochemical studies of DNA behavior in solution.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000427476
0000427475
0000427475
0000427476
0000375495

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Yan Zhang et al.
Nucleosides, nucleotides & nucleic acids, 30(1), 49-62 (2011-01-25)
The Mg(II) complex of Schiff base (K[HL]) derived from vanillin and L-tryptophan could bind with herring sperm DNA. The binding behaviors between them in physiological pH environment (pH 7.40) have been studied by spectroscopy, cyclic voltammetry and viscosity methods. Binding
Po Wang et al.
Biosensors & bioelectronics, 26(7), 3339-3345 (2011-02-08)
A rapid, convenient and accurate method for the simultaneous detection of guanine (G), adenine (A), thymine (T) and cytosine (C) was developed at a multiwalled carbon nanotube (MWCNT)/choline (Ch) monolayer-modified glassy carbon electrode (GCE). X-ray photoelectron spectroscopy data demonstrated that
Synthesis, spectroscopic, crystal structure and DNA binding of Ru(II) complexes with 2-hydroxy-benzoic acid [1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-ethylidene]-hydrazide.
Chitrapriya N, Sathiya Kamatchi T, et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 81, 128-134 (2011)
Mechanistic pathway for controlled extraction of guest molecule bound to herring sperm DNA using α-cyclodextrin.
Jaffer SS, Ghosh P, Purkayastha P.
Spectrochimica Acta Part A: Molecular Spectroscopy, 78, 1587-1591 (2011)
M N Patel et al.
Journal of enzyme inhibition and medicinal chemistry, 26(3), 359-366 (2011-01-29)
A series of ternary copper(II) complexes have been derived using levofloxacin and five phenanthroline derivatives. Complexes were characterized using infrared spectroscopy, Thermogravimetric (TG)-analysis, fast atom bombardment mass spectroscopy and reflectance spectra. Synthesized complexes exhibit the only d-d band at ∼
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Questions

1–5 of 5 Questions  

  1. Is it suitable for melting temperature analysis?

    1 answer

    1. This product has not been tested for melting temperature analysis. The product is not considered to be highly purified. This is an application that will have to be validated by the end user.

      It may be helpful to review the literature. See the links below for publications that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275468/
      https://pubmed.ncbi.nlm.nih.gov/9056205/
      https://www.researchgate.net/publication/313712083_Probing_the_Effects_on_the_Thermal_Stability_of_Salmon_Sperm_DNA_in_the_Presence_of_Metal_Cations_by_Measuring_DNA_Melting_Temperatures

      Helpful?

  2. What is the molecular weight range of Product D3159, Deoxyribonucleic acid?

    1 answer

    1. The molecular weight range is 10,000 - 30,000 Daltons. (One base pair is approximately 660 Daltons.)

      Helpful?

  3. How should I store Product D3159, Deoxyribonucleic acid?

    1 answer

    1. DNA has been stored successfully for several months at 4°C, pH 7.5 - 8, in 10 mM Tris with 1 mM EDTA. The addition of a bacteriostatic agent is not necessary.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. How should I reconstitute Product D3159, Deoxyribonucleic acid?

    1 answer

    1. D3159 is soluble in water at 5 mg per mL. Add slowly to stirring water and then heat gently (60° C or less) or dissolve overnight at 4° C

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