RPMI 8866 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95041316, human blood, Lymphoblastoid

• Synonyme(s) : R8866 Cells, RPMI8866 Cells, Roswell Park Memorial Institute 8866 Cells
• UNSPSC Code: 41106514
• Biological source: human blood
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Not specified
• Morphology: Lymphoblastoid

Propriétés

• biological source: human blood
• packaging: tube of 5 μg 95041316-DNA-5UG, pkg of vial of cells 95041316-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Immunoglobulin E (IgE) binding factor, Calcitonin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Receptor studies, binding assays, hormone studies.

Biochem/physiol Actions

Human B lymphocyte

RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO₂. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.