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RPMI 8866 Cell Line human

95041316, human blood, Lymphoblastoid

Synonyme(s) :

R8866 Cells, RPMI8866 Cells, Roswell Park Memorial Institute 8866 Cells

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About This Item

Code UNSPSC :
41106514
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

RPMI 8866 Cell Line human, 95041316, Lymphoblastoid from blood

Source biologique

human blood

Mode de croissance

Suspension

Caryotype

Not specified

Morphologie

Lymphoblastoid

Produits

Not specified

Récepteurs

Immunoglobulin E (IgE) binding factor, Calcitonin

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−196°C

Catégories apparentées

Origine de la lignée cellulaire

Human B lymphocyte

Description de la lignée cellulaire

RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).

Application

Receptor studies, binding assays, hormone studies.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.

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Questions

1–8 of 8 Questions  

  1. Could you provide information on product 95041316-1VL, including the doubling time and data sheet details?

    1 answer

    1. The RPMI 8866 cell line datasheet is accessible through the cell line catalogue entry on the website. Our ECACC cell culturists have recommended that this cell line might require up to 7 days to reach the exponential or log phase of the cell cycle. Once it reaches this phase, the doubling time of the cell line is expected to be around 18-24 hours, provided the cells are seeded at the optimal subculture seeding density of between 2-3 x 10^4 cells/ml.

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  2. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  3. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  4. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  5. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  6. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  7. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  8. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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