NCI-H727

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94060303, human lung, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lung
• Conditionnement: tube of 5 μg 94060303-DNA-5UG; pkg of vial of cells 94060303-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Synthesise the peptide neuromedin B (NMB) (0.1 pmol/mg protein).
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lung non-small cell carcinoma

Description de la lignée cellulaire

A non-small cell carcinoma of the lung from a 65 year old Caucasian female taken, prior to treatment. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. The cells are not able to synthesise gastrin releasing peptide (GRP). NCI-H727 is the best differentiated available bronchial carcinoid cell line. Any proposed commercial use of these cells or products must first be negotiated with the University of Texas. More details available upon request.

Application

Synthesis of NMB.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 14,15

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.