PGF human cell line

94050342, IHW Number: 9318

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human blood
• Ethnicité: English
• Sexe: male
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Description générale

Consanguineous: Yes

Homozygous: Yes

Description de la lignée cellulaire

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil d'ADN

A: 301
Cw: 7
B: 702
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQA1: 102
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401

Profil sérologique

HLA A: 3
HLA B: 7
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4

Milieu de culture

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Procédure de repiquage

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Clause de non-responsabilité

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.