HCT-15

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91030712, human colon, Epithelial-like

• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46, quasidiploid
• Morphology: Epithelial-like
• Manufacturer/tradename: ECACC

Propriétés

• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 91030712-DNA-5UG, pkg of vial of cells 91030712-1VL
• growth mode: Adherent
• karyotype: 2n = 46, quasidiploid
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

HCT-15 cell line has been used to study the role and efficiency of nexrutine (NX), an extract from Phellodendron amurense, against colon cancer.

Biochem/physiol Actions

Colorectal adenocarcinoma which is tumorigenic in nude mice.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Human colon adenocarcinoma

Preparation Note

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS) (heat inactivated).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.