MDBK Cell Line bovine

90050801, bovine kidney, Epithelial-like

• Synonyme(s) : MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• product name: MDBK Cell Line bovine, 90050801
• Source biologique: bovine kidney
• Mode de croissance: Adherent
• Caryotype: Modal no. 51; hypodiploid
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Bovine kidney

Description de la lignée cellulaire

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

Application

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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