Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89070101, insect ovary, Spherical

• Synonyme(s) : SF-9 Cells
• UNSPSC Code: 41106514
• Biological source: insect ovary
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Spherical
• Manufacturer/tradename: ECACC

Propriétés

• biological source: insect ovary
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89070101-DNA-5UG, pkg of vial of cells 89070101-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Spherical
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.

Biochem/physiol Actions

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.

Spodoptera frugiperda pupal ovarian tissue

Preparation Note

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.