LS180

87021202, human colon, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: LS180, 87021202
• Source biologique: human colon
• Mode de croissance: Adherent
• Caryotype: Modal no. 45 (2n=46)
• Morphologie: Epithelial-like
• Produits: Carcinoembryonic antigen (CEA), Mucin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Application

Tumourigenicity studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Autres remarques

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