3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: 3T3 Swiss Albino, 85022108
• Source biologique: mouse embryo
• Mode de croissance: Adherent
• Caryotype: 2n = 40; hypertriploid
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Product has lost contact inhibition

Origine de la lignée cellulaire

Mouse Swiss Albino embryo fibroblast

Description de la lignée cellulaire

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Application

Contact inhibition and virus studies: polyoma and SV40 viruses

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.