OX-110

13082225, from Rat x Rat Hybridoma

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• Myélome: Y3
• Réactivité: Mouse CD200R
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

The hybridoma cell line OX-110 secretes monoclonal IgG2a antibodies reactive with mouse CD200R.

Immunogène

Recombinant CD200R

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Procédure de repiquage

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ -1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Autres remarques

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.