CAD cell line from mouse

8100805, mouse nerve, Neuronal

• Synonyme(s) : CAD Cell Line
• Code UNSPSC : 41106514

Propriétés

• product name: CAD cell line from mouse, 08100805
• Source biologique: mouse nerve
• Description: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Neuronal
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Description de la lignée cellulaire

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Application

Study of neuronal differentiation

Milieu de culture

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

Autres remarques

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