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C8743

Cholesterol Dehydrogenase from Nocardia sp.

Name: Cholesterol Dehydrogenase from <I>Nocardia</I> sp.
Brand: SIGMA

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A propos de cet article

Numéro CAS:

67775-34-2

MDL number:

MFCD03788777

UNSPSC Code:

12352204

NACRES:

NA.54

Numéro CE :

1.1.1.B40

Specific activity:

≥18 unit/mg solid

Recombinant:

expressed in Streptomyces lividans

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Propriétés

recombinant

expressed in Streptomyces lividans

Quality Segment

200

form

lyophilized solid

specific activity

≥18 unit/mg solid

mol wt

~37 kDa by SDS-PAGE

storage temp.

−20°C

Description

Application

Enzyme used in determining cholesterol in serum

The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.

Biochem/physiol Actions

Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.

Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.

Physical form

Supplied as a lyophilized powder

Other Notes

One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.

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Cet article	C3766	C4405	S0937
Sigma-Aldrich C8743 Cholesterol Dehydrogenase from Nocardia sp.	Sigma-Aldrich C3766 Cholesterol Esterase from bovine pancreas	Sigma-Aldrich C4405 Choline Oxidase from Arthrobacter globiformis	Sigma-Aldrich S0937 Sucrose Phosphorylase
description -	description lyophilized powder, ≥200 units/g protein	description lyophilized powder, 8-20 units/mg solid	description recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid
specific activity ≥18 unit/mg solid	specific activity ≥200 units/g protein	specific activity 8-20 units/mg solid	specific activity ≥45 units/mg solid
recombinant expressed in Streptomyces lividans	recombinant -	recombinant -	recombinant expressed in E. coli
form lyophilized solid	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt ~37 kDa by SDS-PAGE	mol wt 84 kDa by SDS-PAGE	mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration	mol wt 56 kDa by SDS-PAGE
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 + P312 - P501

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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