C7617

Anti-Calnexin antibody, Mouse monoclonal

clone TO-5, purified from hybridoma cell culture

• Synonyme(s) : Anti-CANX, Anti-CNX
• Numéro MDL: MFCD01094399
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TO-5, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~90 kDa
• Espèces réactives: human
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~2 mg/mL
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.
• Isotype: IgG1
• Numéro d'accès UniProt: P27824
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CANX(821)

Description

Description générale

Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca²⁺-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids).

Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca²⁺-dependent retention of proteins.

Spécificité

Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.

Application

Anti-Calnexin antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• flow cytometry
• immunocytochemistry
• immunohistochemistry
• immunofluorescence analysis

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable