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Merck
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Principaux documents

C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

Dissociation Solution

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About This Item

Numéro MDL:
MFCD00282844
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Stérilité

sterile-filtered

Gamme de produits

BioReagent

Forme

liquid

Concentration

1 ×

Technique(s)

cell culture | mammalian: suitable

pH

6.9-7.1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.[1]

Application

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture,[2] U87 glioblastoma tumor cells[3] and cardiac stem cells (CSCs).[4]
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
SLCM7787
SLCK5785
SLCD5242
SLCC1911
SLCC8975

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target
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Questions

1–5 of 5 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 answer

    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

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  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 answer

    1. It will work with most cell types.

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  4. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 answer

    1. Yes, it does contain EDTA; the concentration is proprietary.

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  5. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 answer

    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

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