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C-39165

Sigma-Aldrich

Airway Epithelial Cell Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonyme(s) :

Recommended with C-21260

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Forme

aqueous solution

Conditionnement

pkg of 1 vial

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Airway Epithelial Cell Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-21260

Application

Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages. The medium is optimized for primary human cells, but can also be used for equine, porcine, murine, bat and rat airway epithelial cells.Recommended for:Human Nasal Epithelial Cells (HNEpC)Human Bronchial Epithelial Cells (HBEpC)Human Tracheal Epithelial Cells (HTEpC)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested airway epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Notes préparatoires

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 6.7 ng / mlTransferrin, holo (human): 10 µg / mlRetinoic Acid: 0.1 ng / ml

Stockage et stabilité

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Procédure de repiquage

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Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Primary Airway Epithelial Cells

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

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