C3237

Anti-Caveolin-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-Caveolin-1, Anti-VIP21
• Numéro MDL: MFCD01633097
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 22 kDa
• Espèces réactives: human, rat, mouse
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Technique(s): indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line; microarray: suitable; western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line
• Numéro d'accès UniProt: Q03135
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CAV1(857) ; mouse ... Cav1(12389) ; rat ... Cav1(25404)

Description

Description générale

Anti-Caveolin-1 is developed in rabbit using a synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Caveolin-1 (CAV1) is a multifunctional scaffolding protein, that has three exons. It is located on chromosome 7q31.

Caveolin, a 20-24-kDa integral transmembrane protein, has been identified as a principal component of caveolae membranes. Caveolin (also termed VIP21) exists in several isoforms termed caveolin-1, caveolin-2 and caveolin-3. Caveolin-1 (20-22 kDa) can exist as two isoforms, caveolin-1α and -1β due to alternative splicing of the mRNA.

Spécificité

The sequence is highly conserved in many species, e.g., identical in rat, mouse, bovine, and dog caveolin-1. The sequence is not found in caveolin-2 or caveolin-3.

Immunogène

synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 (amino acids 2-20).

Application

Anti-Caveolin-1 antibody produced in rabbit has been used in:

• indirect immunofluorescence
• in confocal microscopy
• western blotting

Actions biochimiques/physiologiques

Caveolin family members function as scaffolding proteins to organize and concentrate specific lipids such as cholesterol and glycosylphosphatidylinositol (GPI) and lipid modified signaling molecules within caveolae membranes. Staining of caveolin-1 in immunoblotting is specifically inhibited with caveolin-1 immunizing peptide (human, amino acids 2-20).†

Caveolin-1 is expected to play a major role in the growth and evolution of cancer. It participates in glucose metabolism. It may also participate in angiogenesis. Cav-1 helps to control the expression of glucose transporters.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable