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Merck

C-28051

Monocyte Attachment Medium

250 ml

Synonyme(s) :

Primary Cell Culture Media

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A propos de cet article

NACRES:
NA.71
UNSPSC Code:
12352207
Service technique
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product line

PromoCell®

form

aqueous solution

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Monocyte Attachment Medium (Ready-to-use)

Application

The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)

Preparation Note

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Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
The Medium is chemically defined/xeno-free and does not need further supplementation.

Analysis Note

All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Legal Information

PromoCell is a registered trademark of PromoCell GmbH


Classe de stockage

12 - Non Combustible Liquids

Que se passe-t-il ?

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable



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