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C-14092

Sigma-Aldrich

Human Mesenchymal Stem Cells (hMSC)

Derived from adipose tissue, 1 million cells per pellet

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81

Source biologique

human adipose tissue

Conditionnement

pkg of 1,000,000 cells

Morphologie

(stromal)

Application(s)

cell analysis (DNA, RNA, and protein)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Origine de la lignée cellulaire

Adipose Tissue

Application

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Human Mesenchymal Stem Cells (hMSC-AT) are harvested from normal human adipose tissue of individual donors. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. The cells show a verified marker expression profile that complies with ISCT recommendations, providing well characterized cells (Cytotherapy (2006) Vol. 8, No. 4, 315-317).

Qualité

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Forme physique

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Procédure de repiquage

Click here for more information.

Autres remarques

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Informations légales

RNAlater is a registered trademark of Ambion, Inc.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

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Protocoles

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

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