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C0615

Sigma-Aldrich

Cellulase from Trichoderma sp.

greener alternative

powder, ≥5,000 units/g solid

Synonyme(s) :

Onozuka RS

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About This Item

Numéro CAS:
9012-54-8
Numéro de classification (Commission des enzymes):
3.2.1.4 (BRENDA, IUBMB)
Numéro CE :
232-734-4
Numéro MDL:
MFCD00081510
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

powder

Niveau de qualité

200

Activité spécifique

≥5,000 units/g solid

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Solubilité

deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))

Autre catégorie plus écologique

Enabling,

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Manufactured by Yakult
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).

Actions biochimiques/physiologiques

Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences .

Définition de l'unité

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Notes préparatoires

Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Youwen Pan et al.
Applied and environmental microbiology, 76(5), 1433-1441 (2010-01-06)
Biofilm formation by Listeria monocytogenes is generally associated with its persistence in the food-processing environment. Serotype 1/2a strains make up more than 50% of the total isolates recovered from food and the environment, while serotype 4b strains are most often
Rey Allen et al.
Applied and environmental microbiology, 85(7) (2019-02-03)
Phototrophic biofilms are key to nutrient cycling in natural environments and bioremediation technologies, but few studies describe biofilm formation by pure (axenic) cultures of a phototrophic microbe. The cyanobacterium Synechocystis sp. strain PCC 6803 (here Synechocystis) is a model microorganism
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Ninian Prem Prashanth Pabbathi et al.
Biomass conversion and biorefinery, 1-28 (2021-01-14)
As the fossil fuel reserves are depleting rapidly, there is a need for alternate fuels to meet the day to day mounting energy demands. As fossil fuel started depleting, a quest for alternate forms of fuel was initiated and biofuel
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Protocoles

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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