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Merck
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Principaux documents

B9139

Sigma-Aldrich

Biotin-dextran

mol wt 10,000, Lysine-fixable

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About This Item

Numéro MDL:
MFCD00130657
Code UNSPSC :
12352201
eCl@ss :
34058011
Nomenclature NACRES :
NA.25

Conjugué

biotin conjugate

Niveau de qualité

200

Forme

powder

Poids mol.

10,000

Ampleur du marquage

~2 mol per mol

Couleur

white

Solubilité

H2O: soluble 50 mg/mL, clear, colorless to faintly yellow

Température de stockage

2-8°C

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Application

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).

Autres remarques

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000366082
0000366082
0000262383
0000262381
0000267330

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Consulter la Bibliothèque de documents

Victoria L Cressman et al.
The Journal of comparative neurology, 518(14), 2693-2709 (2010-05-28)
Transformations in affective and social behaviors, many of which involve amygdalar circuits, are hallmarks of adolescence in many mammalian species. In this study, using the rat as a model, we provide the first evidence that afferents of the basal amygdala
Jia Liu et al.
Journal of the neurological sciences, 325(1-2), 127-136 (2013-01-16)
The stem cell-based experimental therapies are partially successful for the recovery of spinal cord injury (SCI). Recently, acellular spinal cord (ASC) scaffolds which mimic native extracellular matrix (ECM) have been successfully prepared. This study aimed at investigating whether the spinal
Bao-Ling Du et al.
Brain research, 1400, 87-98 (2011-06-11)
Delivery of cellular and/or trophic factors to the site of injury may promote neural repair or axonal regeneration and return of function after spinal cord injury. Engineered scaffolds provide a platform to deliver therapeutic cells and neurotrophic molecules. To explore
Yang-Can Duan et al.
Pflugers Archiv : European journal of physiology, 459(1), 1-9 (2009-07-25)
This study is to determine whether the nucleus of solitary tract (NTS) is an important component of the central neurocircuitry of the cardiac sympathetic afferent reflex (CSAR) and whether the gamma-aminobutyric acid (GABA) in the NTS modulates the CSAR. Renal
Giam S Vega-Meléndez et al.
Journal of neuroscience research, 92(1), 13-23 (2013-10-30)
Neurotrophins such as ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) and growth factors such as fibroblast growth factor (FGF-2) play important roles in neuronal survival and in axonal outgrowth during development. However, whether they can modulate regeneration after
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