B3640

Anti-Biotine

affinity isolated antibody, lyophilized powder

• Synonyme(s) : Anti-Biotin, Anti Biotin Antibody, Anti Biotin Antibody - Anti-Biotin antibody produced in goat, Anti-Biotin Antibody
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IEP
• Citations: 33

Propriétés

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• technique(s): immunoelectrophoresis: suitable
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

La spécificité et la réactivité sont déterminées par la double diffusion d'Ouchterlony et l'immunoélectrophorèse par rapport au complexe biotine-protéine porteuse. Aucune réactivité n'est observée par rapport à la protéine porteuse.

Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Immunogen

Conjugué biotine-KLH

Application

Dans certaines applications, la localisation de sondes biotinylées avec de l'avidine produit des niveaux de bruit de fond élevés. Des réactifs anti-biotine peuvent être substitués à l'avidine pour diminuer la liaison non spécifique.

Peut être substitué à l'avidine pour diminuer la liaison non spécifique.

Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.

Biochem/physiol Actions

Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.

Physical form

Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2

Preparation Note

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable