Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.[1]
Application
Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.[2]
Reconstitution
Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.
Proceedings of the National Academy of Sciences of the USA, 81.7, 1991-1995 (1984)
CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.
Wang Z, et al.
Methods, 48, 287-293 (2009)
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