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Key Documents

B3148

Sigma-Aldrich

Anticorps monoclonal anti-IgG de chèvre/mouton

clone GT-34, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

biotin conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

secondary antibodies

Clone

GT-34, monoclonal

Forme

buffered aqueous solution

Espèces réactives

sheep, goat, bovine

Ne doit pas réagir avec

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

Technique(s)

direct ELISA: 1:5,000

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

L′anticorps réagit avec un épitope situé sur la chaîne lourde d′IgG1 et IgG2 de chèvre.
Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The product binds to all goat/sheep IgG1

Application

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunoblotting
  • dot blotting
  • immunohistochemistry
  • immunocytochemistry

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.

Actions biochimiques/physiologiques

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Dynamic changes after murine digit amputation: the MRL mouse digit shows waves of tissue remodeling, growth, and apoptosis
Gourevitch DL, et al.
Wound Repair and Regeneration, 17(3), 447 -4455 (2009)
L Li et al.
Scandinavian journal of immunology, 57(5), 410-422 (2003-05-20)
Involution of the thymus and alterations in the development of thymocytes are the most prominent features of age-related immune senescence. We have carried out a comparative analysis of thymocyte and stroma in rapid thymic involution DBA/2 (D2) strain of mice
Sofia Halin Bergström et al.
Scientific reports, 6, 31805-31805 (2016-08-24)
Accumulating data indicates that tumor-derived extracellular vesicles (EVs) are responsible for tumor-promoting effects. However, if tumor EVs also prepare the tumor-bearing organ for subsequent tumor growth, and if this effect is different in low and high malignant tumors is not
Miguel A Ortega et al.
Current issues in molecular biology, 46(5), 3839-3865 (2024-05-24)
Pancreatic cancer is a type of gastrointestinal tumor with a growing incidence and mortality worldwide. Pancreatic ductal adenocarcinoma (PDAC) constitutes 90% of cases, and late-stage diagnosis is common, leading to a 5-year survival rate of less than 10% in high-income
Miguel A Ortega et al.
Journal of cellular and molecular medicine, 24(7), 4157-4170 (2020-03-07)
The development of lower extremity venous insufficiency (VI) during pregnancy has been associated with placental damage. VI is associated with increased oxidative stress in venous wall. We have investigated potential disturbance/dysregulation of the production of reactive oxygen species (ROS) in
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