Accéder au contenu
Merck
Toutes les photos(1)

Documents

APOBRDU

Sigma-Aldrich

Flow Cytometry Kit for Apoptosis

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 60 cell suspensions

Conditionnement

pkg of 1 kit

Technique(s)

flow cytometry: suitable

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Application

The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody.Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. APOBRDU has been used in flow cytometry assay in human non–small cell lung cancer. APOBRDU has been used in cell cycle and apoptosis assay in HeLa cells, and in tunel assay human neuroblastoma cell lines.

Caractéristiques et avantages

Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.

Conditionnement

The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20 °C and APO-PART2 at 2-4 °C

Principe

Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3′-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3′-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.

Composants de kit seuls

Réf. du produit
Description
  • Br-dUTP 480 μL
  • Fluorescein PRB-1 antibody 300 μL
  • Negative control cells 5 mL
  • PI/RNase staining buffer 30 mL
  • Positive control cells 5 mL
  • Reaction buffer .6 mL
  • Rinsing buffer 120 mL
  • Terminal deoxynucleotidyl transferase (TdT) 45 μL
  • Wash buffer 120 mL
Afficher tout (9)

Pictogrammes

FlameHealth hazard
GHS02,GHS08

Mention d'avertissement

Danger

Mentions de danger

H225,H317,H319,H334,H350

Classification des risques

Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

55.4 °F

Point d'éclair (°C)

13 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Colorant vert BioTracker NucView® 488 Caspase-3 (DMSO) Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.

Sigma-Aldrich

SCT100

Colorant vert BioTracker NucView® 488 Caspase-3 (DMSO)

The novel lipophilic camptothecin analogue gimatecan is very active in vitro in human neuroblastoma: a comparative study with SN38 and topotecan
Di Francesco AM, et al.
Biochemical Pharmacology, 70(8), 1125-1136 (2005)
Cardenolide-induced lysosomal membrane permeabilization demonstrates therapeutic benefits in experimental human non-small cell lung cancers
Mijatovic T, et al.
Neoplasia, 8(5), 402-412 (2006)
Cdc7 is an active kinase in human cancer cells undergoing replication stress
Tenca P, et al.
The Journal of Biological Chemistry, 282(1), 208-215 (2007)
X Li et al.
Cell proliferation, 28(11), 571-579 (1995-11-01)
In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing
Xiangjun Sun et al.
Experimental and therapeutic medicine, 17(3), 2334-2340 (2019-03-15)
The present study aimed to elucidate the underlying mechanism of neuroepithelial cell transforming 1 (NET-1), a member of the Ras homolog gene family, in hepatocellular carcinoma (HCC). To determine the association between the expression of NET-1 and the proliferation and

Articles

Tests de viabilité et de prolifération cellulaires

Tests sur cellules pour l'étude de la prolifération (BrdU, MTT, WST1), de la viabilité et de la toxicité cellulaires pour la recherche sur le cancer, la recherche en neurosciences et la recherche sur cellules souches.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique