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APF

Sigma-Aldrich

Alkaline Phosphatase Detection Kit, Fluorescence

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About This Item

Numéro CE :
245-325-0
Code UNSPSC :
12352204
eCl@ss :
42010105
Nomenclature NACRES :
NA.53
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

for molecular biology

Niveau de qualité

200

Utilisation

 kit sufficient for 300 reactions (200 μl reactions)

Technique(s)

microbe id | specific enzyme detection: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... ALPL(249)

Description générale

The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.

Application

Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.[1]
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.

Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Actions biochimiques/physiologiques

Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.[2]

Caractéristiques et avantages

  • Up to 100x more sensitive than colorimetric assays
  • SEAP can be measured without sample destrutcion
  • Rapid and reproducible results
  • Safe and easy-to-use

Composants

The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000186089
029M4140V
11180913
037M4181V
086M4868V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Attila Fábián et al.
Frontiers in plant science, 10, 244-244 (2019-03-23)
As a consequence of climate change, unpredictable extremely hot and dry periods are becoming more frequent during the early stages of reproductive development in wheat (Triticum aestivum L.). Pollen sterility has long been known as a major determinant of fertility
Katharina Besemer et al.
Applied and environmental microbiology, 71(2), 609-620 (2005-02-05)
Natural floodplains play an essential role in the processing and decomposition of organic matter and in the self-purification ability of rivers, largely due to the activity of bacteria. Knowledge about the composition of bacterial communities and its impact on organic-matter
Michael B Dwinell et al.
American journal of physiology. Gastrointestinal and liver physiology, 286(5), G844-G850 (2003-12-20)
Human colonic epithelial cells express CXCR4, the sole cognate receptor for the chemokine stromal cell-derived factor (SDF)-1/CXC chemokine ligand (CXCL) 12. The aim of this study was to define the mechanism and functional consequences of signaling intestinal epithelial cells through
Irena Moserova et al.
PloS one, 7(3), e32972-e32972 (2012-03-10)
We have analyzed the molecular mechanisms of photoinduced cell death using porphyrins with similar structure differing only in the position of the ethylene glycol (EG) chain on the phenyl ring. Meta- and para-positioned EG chains targeted porphyrins to different subcellular
Caterina Bossio et al.
Frontiers in bioengineering and biotechnology, 6, 114-114 (2018-09-14)
Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral
Afficher tous les articles scientifiques apparentés

Questions

1–3 of 3 Questions  

  1. Is Product APF, Alkaline Phosphatase Detection Kit, used for fluorometric or colorimetric assay?

    1 answer

    1. The assay used is fluorometric, which is more sensitive than the colorimetric assay.

      Helpful?

  2. Is Product APF, Alkaline Phosphatase Detection Kit, Fluorescence, used to measure the alkaline phosphatase (AP) activity of cell lysates or the AP activity secreted by cells?

    1 answer

    1. The technical bulletin provides procedures for both. The kit was originally designed for secreted alkaline phosphatase (SEAP), because the human placental SEAP standard that comes with the kit is a secreted enzyme. Its activity in the cell sample can be measured nondestructively and repeatedly over time using an aliquot of the culture medium. It saves time required for cell extract preparations.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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