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Merck
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A9231

Sigma-Aldrich

Acridine Orange solution

2% in H2O

Synonyme(s) :

3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride

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About This Item

Formule empirique (notation de Hill):
C17H19N3 · HCl
Numéro CAS:
65-61-2
Poids moléculaire :
301.81
Numéro MDL:
MFCD00005029
Code UNSPSC :
12171500
ID de substance PubChem :
329771043
Nomenclature NACRES :
NA.47

Forme

liquid

Niveau de qualité

100

Durée de conservation

18 mo.

Concentration

2% in H2O

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

Clé InChI

DPKHZNPWBDQZCN-UHFFFAOYSA-N

Catégories apparentées

Description générale

Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.

Application

Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.

It also has the following applications-
  • staining of RNA and acid mucins
  • detecting apoptosis
  • staining blood smears to diagnose malaria
  • identifying and enumerating granulocytes in fish
  • staining eukaryotic cells to demonstrate low pH structures

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Marko Gerić et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(11), 1521-1527 (2012-06-19)
The present study aimed to characterize chemical properties of clay bricks containing 20 % of sewage sludge. After detection of potentially hazardous metals, we simulated precipitation exposure of such material to determine the amount of heavy metals that could leach
Q L Chen et al.
Talanta, 97, 48-54 (2012-07-31)
We have utilized various valving scheme to leverage purely rotation-regulated flow control to enable comprehensive cell-based bioassays (CBBs) on centrifuge-based lab-on-a-disc (LOAD). A LOAD has been developed to examine allergic degranulation from live basophils for allergens screening for the first
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
Hui-Chuan Yu et al.
PloS one, 8(2), e55705-e55705 (2013-02-06)
Previously, we reported that cancerous inhibitor of protein phosphatase 2A (CIP2A) mediates the apoptotic effect of bortezomib in hepatocellular carcinoma (HCC). Here, we report a proteasome-independent mechanism by which bortezomib induces autophagy in HCC. Our data indicate that bortezomib activated
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