A8311

Antimousse 204

aqueous emulsion for bacterial and mammalian systems

• Synonyme(s) : organic antifoam
• UNSPSC Code: 12161901
• NACRES: NA.85
• MDL number: MFCD00130523

Propriétés

• Quality Segment: 200
• form: emulsion (aqueous)
• concentration: 100% (organic)
• density: 1.01 g/mL at 25 °C
• application(s): microbiology
• storage temp.: room temp
• suitability: (Mammalian (suspension)), bacteria (fermentation)

Description

General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:

• in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
• in fed-batch fermentation
• to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
• in the batch fermentation with mineral medium

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

Other Notes

Ce produit contient 100 % de substances actives ; c'est un mélange d'antimousses organiques non siliconés dans une dispersion polyol. Il peut être stérilisé plusieurs fois.

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves