A8275

Conjugué anticorps anti-IgG de lapin (molécule entière)-peroxydase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Goat Anti-Rabbit IgG (whole molecule)–HRP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162788
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 77

Propriétés

• biological source: goat
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:10,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.

Immunogen

Purified rabbit IgG.

Application

Une co-immunoprécipitation et une analyse par western blot de lysats de cellules C33A ont été réalisées en utilisant de l′IgG anti-lapin de chèvre conjuguée à de la HRP comme anticorps secondaire.

Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.

Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H317,H319,H411
• pcodes: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 3