A6017
ω-Aminohexyl–Agarose
saline suspension
Synonyme(s) :
Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose
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plant
Quality Level
form
saline suspension
extent of labeling
≥5 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino of 1,6-diaminohexane
matrix spacer
1 atom
capacity
≥5 mg/mL binding capacity (bovine serum albumin)
suitability
suitable for chromatography
storage temp.
2-8°C
General description
Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.
Application
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.
ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
Biochem/physiol Actions
ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.
Physical form
Suspension in 0.5 M NaCl containing preservative
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Contenu apparenté
D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
Y Ruttyn et al.
Journal of chromatography, 491(2), 299-308 (1989-07-21)
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition
Hydrophobic chromatography: use for purification of glycogen synthetase.
Shaltiel S and Er-El Z
Proceedings of the National Academy of Sciences of the USA, 70, 778-781 (1973)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| A6017-2.5ML | 04061836971298 |
| A6017-100ML | 04061838219558 |
| A6017-10ML | 04061838219565 |
| A6017-50ML | 04061833376805 |
| A6017-1ML | 04061836971281 |