A2264
β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)
ammonium sulfate suspension, ≥10 units/mg protein
Synonyme(s) :
β-N-Acetylhexosaminidase
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About This Item
Produits recommandés
Source biologique
Canavalia ensiformis
Niveau de qualité
Forme
ammonium sulfate suspension
Activité spécifique
≥10 units/mg protein
Activité étrangère
α- and β-galactosidase, and α-L-fucosidase ≤0.1%
α-mannosidase ≤0.2%
Température de stockage
2-8°C
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Application
β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .
Actions biochimiques/physiologiques
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.
Forme physique
Suspension in 2.5 M (NH4)2SO4, pH 7.0
Remarque sur l'analyse
At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.
Inhibiteur
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Journal of medical microbiology, 55(Pt 8), 999-1008 (2006-07-20)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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