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A1543

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Goat Anti-Human IgG (whole molecule)−AP

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About This Item

Numéro MDL:
MFCD00162453
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

goat

Niveau de qualité

200

Produit recombinant

expressed in goat

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:20,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Immunogène

IgG purified from human serum

Application

Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.
Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37°C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000341554
0000328714
0000329427
0000341554
0000328714

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Molouk Beiromvand et al.
Journal of infection in developing countries, 13(2), 154-161 (2020-02-10)
Cystic Echinococcosis (CE) and toxocariasis caused by the larval stages of intestinal dog worms including Echinococcus granulosus and Toxocara spp. are among the most widespread zoonotic diseases. Four hundred municipal waste collectors were serologically evaluated for CE and toxocariasis. To
C T Ravirajan et al.
Rheumatology (Oxford, England), 43(4), 442-447 (2004-03-17)
There is considerable evidence suggesting that anti-double-stranded deoxyribonucleic acid (anti-dsDNA) antibodies are involved in the pathogenesis of lupus nephritis. It was shown previously using severe combined immune deficient (SCID) mice that when the hybridomas secreting human immunoglobulin G (IgG) anti-dsDNA
Jens Bukh et al.
Journal of medical virology, 80(1), 87-94 (2007-11-28)
GB virus-B (GBV-B) is the virus most closely related to hepatitis C virus (HCV). Thus, we have used GBV-B infection of tamarins, which develop acute hepatitis following experimental infection, as a surrogate model to study protective immunity. As challenge virus
Jessica E Manning et al.
The Journal of infectious diseases, 226(8), 1327-1337 (2021-11-01)
We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins. Children aged 2-9 years were enrolled in the PAGODAS (Pediatric Assessment Group of
L Lledó et al.
Journal of medical microbiology, 51(10), 861-865 (2002-11-19)
Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and
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Questions

1–3 of 3 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the source of the alkaline phosphatase that is used to make Product A1543, Anti-Human IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat?

    1 answer

    1. Product A1543, Anti-Human IgG (whole molecule) -Alkaline Phosphatase antibody produced in goat, is prepared using alkaline phosphatase from bovine intestinal mucosa.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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