84120

Safranine O

for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)

• Synonyme(s) : Basic Red 2, Cotton Red, Gossypimine, Safranine T, Safranine Y ou A
• Formule empirique (notation de Hill): C₂₀H₁₉ClN₄
• Numéro CAS: 477-73-6
• Poids moléculaire : 350.84
• Numéro C.I. (Colour Index): 50240
• Numéro Beilstein : 3924099
• Numéro CE : 207-518-8
• Numéro MDL: MFCD00011759
• Code UNSPSC : 12171500
• ID de substance PubChem : 329769290
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy (Bact., Bot., Hist.); indicator (pH 0.3-1.0)
• Forme: powder or crystals
• Technique(s): titration: suitable
• pH: 10 (20 °C, 10 g/L)
• Solubilité: methanol: 0.01 g/10 mL, red
• εmax: ≥1000 at 525-535 nm in 50% ethanol
• Application(s): diagnostic assay manufacturing; hematology; histology; microbiology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• Clé InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Description

Description générale

Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Application

• Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
• It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
• It is a component of Benda′s polychrome stain.
• It is a component of various polychromes and lignin stains for botanical staining applications.
• It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves