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83695

Sigma-Aldrich

Rhodamine 110 chloride

suitable for fluorescence, BioReagent, ≥99.0% (UV)

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About This Item

Formule empirique (notation de Hill):
C20H14N2O3 · HCl
Numéro CAS:
13558-31-1
Poids moléculaire :
366.80
Numéro Beilstein :
4631860
Numéro CE :
236-944-7
Numéro MDL:
MFCD00042009
Code UNSPSC :
12171500
ID de substance PubChem :
57652964
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥99.0% (UV)

Forme

crystals

Pf

>300 °C (lit.)

Solubilité

ethanol: soluble
methanol: soluble

Fluorescence

λex 498 nm; λem 520 nm in methanol

Adéquation

suitable for fluorescence

Chaîne SMILES 

Cl.Nc1ccc2c(OC3=CC(=N)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H

Clé InChI

JNGRENQDBKMCCR-UHFFFAOYSA-N

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Description générale

Rhodamine 110 or R110 is a triphenylmethane dye and emits a strong green-fluorescent light. The high fluorescence is attributed to the presence of amino groups as electron donating groups. Rhodamine 110 has excitation and emission maximum at 502.4/521.6 nm, respectively.

Application

Rhodamine 110 is used for the determination of protease activity with accurate kinetic parameters. It is used to measure caspase activity in cells with induced apoptosis.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Xian Zhang et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 59(8), 1667-1672 (2003-05-09)
A simple and sensitive fluorescence quenching method for the determination of trace nitrite has been developed. The method is based on the reaction of Rhodamine 110 with nitrite in acidic medium to form a new compound, which has much lower
Ana Virel et al.
Analytical chemistry, 84(5), 2380-2387 (2012-02-14)
By site directed mutagenesis, we have produced recombinant mutants of human and mouse prethrombin-2 which are able to convert themselves autocatalytically into α-thrombin. We also have created a new method to amplify the signal of bioanalytical assays based on the
Stanislav Kalinin et al.
The Review of scientific instruments, 83(9), 096105-096105 (2012-10-02)
We present a fast hardware photon correlator implemented in a field-programmable gate array (FPGA) combined with a compact confocal fluorescence setup. The correlator has two independent units with a time resolution of 4 ns while utilizing less than 15% of
Simeonika Rangełowa-Jankowska et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(13), 2449-2452 (2011-07-14)
The first observation of strong directional surface-plasmon-coupled emission (SPCE) of Rhodamine 110 in silica nanofilms deposited on silver nanolayers is reported. The preparation of the material is described in detail. The intensity of SPCE exceeds 10 times that of free
Tatyana G Terentyeva et al.
Bioconjugate chemistry, 22(10), 1932-1938 (2011-09-13)
Commonly used fluorogenic substrate analogues for the detection of protease activity contain two enzyme-cleavable bonds conjugated to the fluorophore. Enzymatic cleavage follows a two-step reaction with a monoamide intermediate. This intermediate shows fluorescence at the same wavelength as the final
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