71183

Acetate de sodium

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• Synonyme(s) : Acide acétique sodium salt
• Formule linéaire : CH₃COONa
• Numéro CAS: 127-09-3
• Poids moléculaire : 82.03
• Numéro Beilstein : 3595639
• Numéro CE : 204-823-8
• Numéro MDL: MFCD00012459
• Code UNSPSC : 12161700
• eCl@ss : 39021906
• ID de substance PubChem : 57652226
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: for luminescence; for molecular biology
• Gamme de produits: BioUltra
• Pureté: ≥99.0% (NT)
• Forme: solid
• Température d'inflammation spontanée: 1112 °F
• Impuretés: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• Perte: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• Pf: >300 °C (dec.) (lit.)
• Solubilité: H₂O: 3 M at 20 °C, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• Traces de cations: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• Chaîne SMILES: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• Absorption UV: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• Clé InChI: VMHLLURERBWHNL-UHFFFAOYSA-M

Description

Application

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

Actions biochimiques/physiologiques

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Autres remarques

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)