51435C

Milieu d′Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose

DMEM/High Modified, with 4500 mg/L dextrose, with 110 mg/L sodium pyruvate, without ferric nitrate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : DME, DMEM
• NACRES: NA.75
• UNSPSC Code: 12352207

Propriétés

• Quality Segment: 500
• description: for research or for further manufacturing use
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable, cell culture | stem cell: suitable
• components: L-glutamine: no; glucose: high; phenol red: yes; sodium pyruvate: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium has high concentration of amino acids and vitamins.

Application

Le milieu de Eagle modifié par Dulbecco (DMEM) est une variante du milieu de Eagle de base (BME) qui contient quatre fois plus d'acides aminés et de vitamines. La formulation d'origine contenait 1 000 mg/l de glucose et servait à la culture de cellules embryonnaires de souris. Depuis, le milieu a été modifié à plusieurs reprises pour convenir aux primocultures de cellules de souris et de poulet ainsi que de diverses cellules normales ou transformées. Chacun de ces milieux apporte une combinaison différente de L-glutamine et de pyruvate de sodium. En outre, le taux de glucose a été augmenté à 4 500 mg/l, d'où le nom "DMEM/High" pour DMEM riche en glucose.

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s modified eagle′s medium/high modified has been used in the characterisation of nanoparticles in cell culture media.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable