PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Synonyme(s) : 10X TE buffer
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Gamme de produits: pHast Pack^™
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: ≤5 ppm Heavy Metals (lead)
• Couleur: white
• pH: 7.9—8.1
• Solubilité: soluble, clear, colorless
• Traces de cations: Fe: <10 ppm
• Activité étrangère: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Température de stockage: room temp

Description

Description générale

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Application

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Caractéristiques et avantages

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Conditionnement

Foil pouch

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Stockage et stabilité

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations légales

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H373
• Conseils de prudence: P260 - P314 - P501
• Classification des risques: STOT RE 2 Inhalation
• Organes cibles: Respiratory Tract
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable