This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.
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| Taille/Référence | Disponibilité | Prix |
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A propos de cet article
biological source
bovine
Quality Segment
form
liquid
contains
0.05% sodium azide as preservative
packaging
ampule of 1 mL
concentration
0.98—1.02 mg protein/mL
technique(s)
ELISA: suitable
color
clear colorless
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
- comme étalon dans la quantification de la libération de protéines par la méthode BCA (à l'acide bicinchoninique).
- comme étalon dans la quantification de l'urée par le test de Bradford.
- comme marqueur de taille moléculaire pour la quantification de protéines par électrophorèse sur gel de polyacrylamide en conditions natives.
Packaging
Other Notes
1 of 1
Cet article | |||
|---|---|---|---|
| description Micro Standard, liquid | description liquid, 2 mg protein/ml | description analytical standard, 200 mg/mL (BSA) | description analytical standard, 80 mg/mL (HSA and gamma-globulins) |
| biological source bovine | biological source bovine | biological source - | biological source human |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) gel permeation chromatography (GPC): suitable | technique(s) gel permeation chromatography (GPC): suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form - | form liquid |
| Gene Information bovine ... ALB(280717) | Gene Information bovine ... ALB(280717) | Gene Information - | Gene Information - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Classe de stockage
12 - Non Combustible Liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?
1 answer-
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What is the shipping temperature?
1 answer-
The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.
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Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?
1 answer-
The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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