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69385

Supelco

Protein Standard Mix 15 - 600 kDa

for size exclusion chromatography

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About This Item

Code UNSPSC :
85151701
Nomenclature NACRES :
NA.24

Niveau de qualité

100

Forme

solid

Poids mol.

15-600 kDa

Classe(s) chimique(s) de l'analyte

amino acids, peptides, proteins

Technique(s)

gel permeation chromatography (GPC): suitable

Application(s)

clinical
food and beverages
pharmaceutical

Format

neat

Température de stockage

−20°C

Description générale

The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.

Components

Thyroglobulin bovine MW ~ 670 000 Da

γ-globulins from bovine blood MW ~ 150 000 Da

Ovalbumin MW~ 44 300 Da

Ribonuclease A type I-A MW ~ 13 700 Da

p-aminobenzoic acid (pABA) MW ~ 137 Da

Application

This analytical standard is used for the following:
  • Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column
  • Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD)
  • Molecular weight separation of proteins by size-exclusion chromatography, formed upon O3 and NO2 induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein
  • Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

1445550

Compatibilité aux systèmes d′IgG monoclonaux, United States Pharmacopeia (USP) Reference Standard

80023448

Colonne HPLC TSKgel® UP-SW3000, phase diol, L × I.D. 30 cm × 4.6 mm, 2 μm particle size

Z777300

Unix™ SEC-300 UHPLC Column, L × I.D. 15 cm × 4.6 mm, 1.8 μm particle size

Z777302

Unix™ SEC-300 UHPLC Column, L × I.D. 30 cm × 4.6 mm, 1.8 μm particle size

1445595

USP mAb 003, Monoclonal IgG1, United States Pharmacopeia (USP) Reference Standard

1445539

USP mAb 001, Monoclonal IgG1, United States Pharmacopeia (USP) Reference Standard

1445547

USP mAb 002, Monoclonal IgG1, United States Pharmacopeia (USP) Reference Standard

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
BCCL2045
BCCK8152
BCCL8413
BCCL5372
BCCK1855

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Thomas Fricke et al.
Viruses, 14(3) (2022-03-27)
Kaposi's sarcoma herpesvirus (KSHV) is associated with a significant disease burden, in particular in Sub-Sahara Africa. A KSHV vaccine would be highly desirable, but the mechanisms underlying neutralizing antibody responses against KSHV remain largely unexplored. The complex made of glycoproteins
Christopher J Kampf et al.
Environmental science & technology, 49(18), 10859-10866 (2015-08-20)
Air pollution is a potential driver for the increasing prevalence of allergic disease, and post-translational modification by air pollutants can enhance the allergenic potential of proteins. Here, the kinetics and mechanism of protein oligomerization upon ozone (O3) exposure were studied
Robert C Monsen et al.
Nucleic acids research, 49(3), 1749-1768 (2021-01-21)
Human telomeres contain the repeat DNA sequence 5'-d(TTAGGG), with duplex regions that are several kilobases long terminating in a 3' single-stranded overhang. The structure of the single-stranded overhang is not known with certainty, with disparate models proposed in the literature.
Amberley D Stephens et al.
Analytical chemistry, 90(11), 6975-6983 (2018-05-12)
Understanding the mechanisms behind amyloid protein aggregation in diseases, such as Parkinson's and Alzheimer's disease, is often hampered by the reproducibility of in vitro assays. Yet, understanding the basic mechanisms of protein misfolding is essential for the development of novel
Anastasiya Lavell et al.
The Plant journal : for cell and molecular biology, 108(5), 1332-1345 (2021-09-29)
Rhomboid-like proteins are intramembrane proteases with a variety of regulatory roles in cells. Though many rhomboid-like proteins are predicted in plants, their detailed molecular mechanisms or cellular functions are not yet known. Of the 13 predicted rhomboids in Arabidopsis thaliana
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