63184

5-Maleimido-eosin

suitable for fluorescence, ≥93% (HPLC)

• Synonyme(s) : EMA, Eosin-5-maleimide
• Formule empirique (notation de Hill) : C₂₄H₉Br₄NO₇
• Numéro CAS: 150322-02-4
• Poids moléculaire : 742.95
• NACRES: NA.32
• PubChem Substance ID: 329759808
• UNSPSC Code: 12352207
• MDL number: MFCD07437936

Propriétés

• Quality Segment: 100
• assay: ≥93% (HPLC)
• form: powder
• mol wt: 742.95 g/mol
• technique(s): protein detection: suitable
• color: light orange to dark orange, light red
• fluorescence: λ_ex 524 nm; λ_em 545 nm (after derivatization with 2-mercaptoethanol)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br
• InChI: 1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H
• InChI key: YRCLWXRIEYQTAV-UHFFFAOYSA-N

Description

General description

5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.

Application

Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).

Other Notes

Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)