28514

Methylene Blue hydrate

≥95% (calc. to the dried substance)

• Synonyme(s) : Basic Blue 9 monohydrate ; Methylene Blue (hydrate)
• Formule empirique (notation de Hill) : C₁₆H₁₈ClN₃S · xH₂O
• Numéro CAS: 122965-43-9
• Poids moléculaire : 319.85 (anhydrous basis)
• UNSPSC Code: 12171500
• NACRES: MA.02
• PubChem Substance ID: 329753217
• EC Number: 200-515-2
• Beilstein/REAXYS Number: 3599847
• MDL number: MFCD00150006

Propriétés

• Quality Segment: 200
• assay: ≥95% (calc. to the dried substance)
• form: powder
• impurities: ≤1% insoluble in ethanol
• loss: ≤22% loss on drying, 105 °C
• color: very dark green
• solubility: ethanol: insoluble ≤1%
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• InChI key: WQVSELLRAGBDLX-UHFFFAOYSA-M

Description

General description

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C₁₆H₁₈N₃SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Application

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:

• in the preparation of methylene blue (MB) solution
• to stain zymograms -to label the injection site in the tissue slices
• to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Informations sur la sécurité

• Pictogrammes: GHS07
• Mot-clé: Warning
• Codes de danger: H302
• Mentions de précaution: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): dust mask type N95 (US), Eyeshields, Gloves