DCTP-RO

dCTP

99% (dNTP), <0.9% (dNDP), solution, 100 mM

• Synonyme(s) : 2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na₂
• Formule empirique (notation de Hill): C₉H₁₄N₃Na₂O₁₃P₃
• Numéro CAS: 102783-51-7
• Poids moléculaire : 511.12
• Numéro MDL: MFCD00084683
• Code UNSPSC : 41106305
• ID de substance PubChem : 329798916

Propriétés

• Description: PCR Grade, sodium salt
• Niveau de qualité: 100
• Pureté: 99% (dNTP)
• Forme: solution
• Utilisation: sufficient for 125,000 standard reactions (03732690001); sufficient for 31,250 standard reactions (11969021001); sufficient for 6,250 standard reactions (11934520001)
• Conditionnement: pkg of 1,250 μL (11969021001 [100 mM]); pkg of 4 × 1,250 μL (03732690001 [4x100 mM]); pkg of 250 μL (11934520001 [100 mM])
• Fabricant/nom de marque: Roche
• Concentration: <0.9% (dNDP); 100 mM
• Température de stockage: −20°C
• Chaîne SMILES: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• Clé InChI: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Description

Description générale

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Qualité

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Forme physique

solution of dCTP in water (pH 8.3).

Notes préparatoires

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Autres remarques

For general laboratory use.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash