AGRLE-RO

Agarose LE

low electroendosmosis

• Synonyme(s) : agarose
• Code UNSPSC : 12352200

Propriétés

• Source biologique: algae (seaweed)
• Forme: powder
• Conditionnement: pkg of 100 g (11685660001); pkg of 500 g (11685678001)
• Fabricant/nom de marque: Roche
• EEO: 0.05 -0.13
• Température de transition: gel point 36 °C ±1.5
• Résistance du gel: >=2,500 g/cm²
• Activité étrangère: Dnase, none detected; Rnase, none detected
• Conditions d'expédition: ambient
• Température de stockage: 20-25°C

Description

Description générale

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

• analyzing fragments between 0.2 and 15 kb
• analysis of PCR products
• examination of restriction endonucleases
• digests of plasmid, cosmid, and λ phage DNA
• electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
• in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Qualité

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash