11585878001
Roche
hGH ELISA
sufficient for 192 tests, >98% (SDS-PAGE), kit of 1, suitable for ELISA
Synonyme(s) :
ELISA
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41116158
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Essai
>98% (SDS-PAGE)
Utilisation
sufficient for 192 tests
Conditionnement
kit of 1
Fabricant/nom de marque
Roche
Technique(s)
ELISA: suitable
Température de stockage
2-8°C
Description générale
Human growth hormone (hGH) is specifically detected by the hGH ELISA. It does not cross-react with rat growth hormone. Cross-reactivity with the human homologues TSH, FSH, and LH has not been tested. The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
hGH Standards
The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
hGH Standards
The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g., RNA isolation). Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells.
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.
Spécificité
The hGH ELISA specifically detecs human growth hormone (hGH). It does not cross-react with rat growth hormone.
Application
Caractéristiques et avantages
- Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used
- More sensitive: The hGH ELISA is approximately 20-times more sensitive than isotopic hGH assays
- More specific: The hGH ELISA shows no crossreactivity with rat growth hormone
- Faster: Measures hGH expression as early as 18hours post-transfection and requires only approximately 4 hours from start to finish
- Easy to perform: Follows a standard ELISA protocol
Conditionnement
1 kit containing 9 components.
Caractéristiques
Assay time: Approximately 4 hours
Sample material: Culture supernatant
Sensitivity: ≥5pg/ml (≥1pg/well)
Standards: The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
Sample material: Culture supernatant
Sensitivity: ≥5pg/ml (≥1pg/well)
Standards: The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
Principe
The hGH ELISA is based on the sandwich ELISA principle. Antibodies to hGH (anti-hGH) are prebound to the surface of the microplate. The culture supernatant, which contains secreted hGH, is added to the wells of the microplate modules. All hGH contained in the medium binds to the anti-hGH bound to the microplate surface. Next, a digoxigenin-labeled antibody to hGH (anti-hGH-DIG) is added, then bound to hGH. In the following step, an antibody to digoxigenin conjugated to peroxidase (anti-DIG-POD) is added, then bound to digoxigenin. In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate, yielding a colored reaction product. The absorbance of the sample is determined using an ELISA reader, and is directly correlated to the level of hGH present in the medium supernatant. The sensitivity of the assay can be enhanced using the peroxidase substrate (ABTS) with substrate enhancer.
Notes préparatoires
Working solution: Solution 1 hGH Stock Solution (bottle 1)
Reconstitute the lyophilizate in 0.5 ml double distilled water. The resulting concentration is calculated using the lot-specific information, given on the label (final conc. approx 10 ng/ml). This solution is used for the preparation of hGH standards for establishing a hGH calibration curve (see 7.2: preparation of hGH standards).
Solution 2 Anti-hGH-DIG (bottle 2)
Reconstitute the lyophilizate in 0.5 ml double distilled water (final conc. 100 μg/ml).
Solution 2a Anti-hGH-DIG, working dilution
To prepare anti-hGH-DIG working dilution, dilute the reconstituted anti-hGH-DIG solution (100 g/ml) with sample buffer (solution 7) to a final conc. of 1 μg/ml (e.g., 100 μl of reconstituted anti-hGH-DIG solution 9.9 ml of solution 7 for 50 wells).The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
Reconstitute the lyophilizate in 0.5 ml double distilledwater (final conc. 20 U/ml). Do not add sodiumazide!
Solution 3a Anti-DIG-POD, working dilution
To prepare anti-DIG-POD, working dilution, dilute the reconstituted anti-DIG-POD solution (20 U/ml) with sample buffer (solution 7) to a final conc. of 200 mU/ml (e.g., 100 μl of reconstituted anti-DIG-POD solution 9.9 ml of solution 7 for 50 wells). The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
Ready-to-use solution.
Solution 5 POD Substrate containing Substrate Enhancer (bottles 4 and 5)
If a low hGH concentration in the sample is expected, add 1 mg of substrate enhancer (bottle 5) per ml of solution 4 and mix by stirring for 30 min at 15 to 25 °C. The solution is stable for only 2 hours and should therefore be prepared immediately before use. Use the substrate enhancer only if the hGH concentration is low!
Solution 6 Washing Buffer, 1× (bottle 6)
To prepare a ready-to-use washing buffer, mix 1 part 10x washing buffer concentrate (bottle 6) with 9 parts of double distilled water.
Solution 7 Sample Buffer (bottle 7)
The solution is ready-to-use. Mix thoroughly before use. Do not add sodium azide.
Storage conditions (working solution): Solution 1 hGH Stock Solution (bottle 1)
The reconstitutedsolution is stable for 2 months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2 Anti-hGH-DIG (bottle 2)
The reconstituted solution is stable for two months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2a Anti-hGH-DIG, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
The reconstituted solution is stable for 6 months at 2 to 8 °C. Do not freeze!
Solution 3a Anti-DIG-POD, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
The solution is stable until the expiration date given on the label if stored at 2 to 8 °C.
POD Substrate containing Substrate Enhancer (bottles 4 and 5)
The solution is stable for only 2 hours and should therefore be prepared immediately before use.
Solution 6 Washing Buffer, 1× (bottle 6)
The reconstituted solution is stable for 6 months at 2 to 8 °C.
Solution 7 Sample Buffer (bottle 7)
After opening the bottle we recommend storing the solution in aliquots at -15 to -25 °C, since it does not contain a preservative agent.
Reconstitute the lyophilizate in 0.5 ml double distilled water. The resulting concentration is calculated using the lot-specific information, given on the label (final conc. approx 10 ng/ml). This solution is used for the preparation of hGH standards for establishing a hGH calibration curve (see 7.2: preparation of hGH standards).
Solution 2 Anti-hGH-DIG (bottle 2)
Reconstitute the lyophilizate in 0.5 ml double distilled water (final conc. 100 μg/ml).
Solution 2a Anti-hGH-DIG, working dilution
To prepare anti-hGH-DIG working dilution, dilute the reconstituted anti-hGH-DIG solution (100 g/ml) with sample buffer (solution 7) to a final conc. of 1 μg/ml (e.g., 100 μl of reconstituted anti-hGH-DIG solution 9.9 ml of solution 7 for 50 wells).The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
Reconstitute the lyophilizate in 0.5 ml double distilledwater (final conc. 20 U/ml). Do not add sodiumazide!
Solution 3a Anti-DIG-POD, working dilution
To prepare anti-DIG-POD, working dilution, dilute the reconstituted anti-DIG-POD solution (20 U/ml) with sample buffer (solution 7) to a final conc. of 200 mU/ml (e.g., 100 μl of reconstituted anti-DIG-POD solution 9.9 ml of solution 7 for 50 wells). The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
Ready-to-use solution.
Solution 5 POD Substrate containing Substrate Enhancer (bottles 4 and 5)
If a low hGH concentration in the sample is expected, add 1 mg of substrate enhancer (bottle 5) per ml of solution 4 and mix by stirring for 30 min at 15 to 25 °C. The solution is stable for only 2 hours and should therefore be prepared immediately before use. Use the substrate enhancer only if the hGH concentration is low!
Solution 6 Washing Buffer, 1× (bottle 6)
To prepare a ready-to-use washing buffer, mix 1 part 10x washing buffer concentrate (bottle 6) with 9 parts of double distilled water.
Solution 7 Sample Buffer (bottle 7)
The solution is ready-to-use. Mix thoroughly before use. Do not add sodium azide.
Storage conditions (working solution): Solution 1 hGH Stock Solution (bottle 1)
The reconstitutedsolution is stable for 2 months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2 Anti-hGH-DIG (bottle 2)
The reconstituted solution is stable for two months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2a Anti-hGH-DIG, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
The reconstituted solution is stable for 6 months at 2 to 8 °C. Do not freeze!
Solution 3a Anti-DIG-POD, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
The solution is stable until the expiration date given on the label if stored at 2 to 8 °C.
POD Substrate containing Substrate Enhancer (bottles 4 and 5)
The solution is stable for only 2 hours and should therefore be prepared immediately before use.
Solution 6 Washing Buffer, 1× (bottle 6)
The reconstituted solution is stable for 6 months at 2 to 8 °C.
Solution 7 Sample Buffer (bottle 7)
After opening the bottle we recommend storing the solution in aliquots at -15 to -25 °C, since it does not contain a preservative agent.
Remarque sur l'analyse
No cross-reaction with rat growth hormone. Cross reactivity with the human homologues TSH, FSH, and LH has not been tested.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Composants de kit seuls
Réf. du produit
Description
- hGH (human growth hormone)
- Anti-hGH-digoxigenin (anti-hGH-DIG) antibody, lyophilizate
- Anti-Digoxigenin-POD antibody, lyophilizate
- POD Substrate ready-to-use
- Substrate Enhancer
- Washing Buffer 10x concentrated
- Sample Buffer
- Microplates (24x8 wells), precoated with anti-hGH (2)
- Self-adhesive Plate Cover Foils (6)
Afficher tout (9)
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Gabriela da Silva Xavier et al.
The Biochemical journal, 371(Pt 3), 761-774 (2003-02-19)
AMP-activated protein kinase (AMPK) has recently been implicated in the control of preproinsulin gene expression in pancreatic islet beta-cells [da Silva Xavier, Leclerc, Salt, Doiron, Hardie, Kahn and Rutter (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 4023-4028]. Using pharmacological and
Jessica R Chaffey et al.
Scientific reports, 11(1), 15624-15624 (2021-08-04)
The generation of a human pancreatic beta cell line which reproduces the responses seen in primary beta cells, but is amenable to propagation in culture, has long been an important goal in diabetes research. This is particularly true for studies
Woo-Jae Park et al.
Neuroscience letters, 444(3), 240-244 (2008-09-02)
Leucine-rich glioma inactivated 3 (LGI3) is a member of LGI/epitempin (EPTP) family. The biological function of LGI3 and its association with disease are not known. We previously reported that mouse LGI3 was highly expressed in brain in a developmentally and
Chelsea E Stamm et al.
mSphere, 4(3) (2019-06-07)
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is one of the most successful human pathogens. One reason for its success is that Mtb can reside within host macrophages, a cell type that normally functions to phagocytose and destroy infectious
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
