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11585614910

Roche

DIG-High Prime DNA Labeling and Detection Starter Kit II

greener alternative

sufficient for 12 labeling reactions (10 ng to 3 μg per assay), sufficient for 24 blots (blots of 100 cm2)

Synonyme(s) :

DIG system, dna labeling

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About This Item

Code UNSPSC :
41105500

Utilisation

sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
 sufficient for 24 blots (blots of 100 cm2)

Niveau de qualité

100

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Autre catégorie plus écologique

Aligned,

Température de stockage

−20°C

Description générale

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:
  • in Southern blots
  • in northern blots
  • in dot blots
  • in colony and plaque hybridizations
  • for all types of filter hybridization
  • for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Conditionnement

1 kit containing 7 components.

Principe

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • DIG-High Prime 5x concentrated
  • DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml
  • DNA Dilution Buffer
  • Anti-Digoxigenin-AP Conjugate antibody
  • CSPD ready-to-use
  • Blocking Solution 10x concentrated
  • DIG Easy Hyb Granules

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
59457520
56477220
35941221
35941220
27394020

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Erum Dilshad et al.
PloS one, 10(10), e0140266-e0140266 (2015-10-09)
The potent antimalarial drug artemisinin has a high cost, since its only viable source to date is Artemisia annua (0.01-0.8% DW). There is therefore an urgent need to design new strategies to increase its production or to find alternative sources.
Ling Zhang et al.
International journal of genomics, 2014, 921950-921950 (2014-09-10)
The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by
Eva-Maria Holstein et al.
Cell reports, 7(4), 1259-1269 (2014-05-20)
A large and diverse set of proteins, including CST complex, nonsense mediated decay (NMD), and DNA damage response (DDR) proteins, play important roles at the telomere in mammals and yeast. Here, we report that NMD, like the DDR, affects single-stranded
M Dutt et al.
Plant cell reports, 26(12), 2101-2110 (2007-08-19)
Shoot apical meristem explants of Vitis vinifera "Thompson Seedless" were used for Agrobacterium-mediated genetic transformation. It was determined that the meristems had to be subjected to a dark growth phase then wounded to obtain transgenic plants. Morphological and histological studies
Bushra Hafeez Kiani et al.
Malaria journal, 15(1), 252-252 (2016-05-05)
Malaria is causing more than half of a million deaths and 214 million clinical cases annually. Despite tremendous efforts for the control of malaria, the global morbidity and mortality have not been significantly changed in the last 50 years. Artemisinin, extracted
Afficher tous les articles scientifiques apparentés

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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