11333054001

ester de NHS-digoxigénine

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• Synonyme(s) : εAcide ε-digoxigénine-3-0-acétamido)caproïque, ester de N-hydroxysulfosuccinimide, DIG NHS
• Formule empirique (notation de Hill): C₃₅H₅₀N₂O₁₀
• Numéro CAS: 129273-26-3
• Poids moléculaire : 658.78
• Numéro MDL: MFCD02093394
• Code UNSPSC : 41105600
• ID de substance PubChem : 329749387

Propriétés

• Pureté: >90% (elementary analysis)
• Forme: powder
• Poids mol.: M_r 658.8
• Conditionnement: pkg of 5 mg
• Fabricant/nom de marque: Roche
• Plage de pH: 2-13
• Solubilité: DMF: soluble; DMSO: soluble; ethanol: soluble
• Température de stockage: −20°C
• Chaîne SMILES: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• Clé InChI: KHNDABJZSPPYLE-FUGFVFQCSA-N

Description

Description générale

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Application

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Actions biochimiques/physiologiques

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Principe

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Notes préparatoires

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H300,H319,H331,H373,H410
• Conseils de prudence: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Classification des risques: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable