RADPCMAG-82K

MILLIPLEX^® Rat Adipocyte Panel - Metabolism Assay

• Code UNSPSC : 12161503
• eCl@ss : 32161000
• Nomenclature NACRES : NA.84

Propriétés

• Espèces réactives: rat
• Niveau de qualité: 200
• Fabricant/nom de marque: Milliplex^®
• assay range: accuracy: 94-121%; sensitivity: 0.1-25 pg/mL; (MinDC+2SD); standard curve range: 0.7-3,000 pg/mL; (IL-1β, TNFα); standard curve range: 12-50,000 pg/mL; (PAI-1); standard curve range: 122-500,000 pg/mL; (Adiponectin); standard curve range: 15-60,000 pg/mL; (IL-6, Leptin); standard curve range: 9.8-40,000 pg/mL; (MCP-1)
• Technique(s): multiplexing: suitable
• Méthode de détection: fluorometric (Luminex xMAP)
• Conditions d'expédition: wet ice

Description

Description générale

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.

The MILLIPLEX^® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

The MILLIPLEX^® MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα.

Spécificité

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
• Recommended Sample Type: Rat cell/tissue culture supernatant or lysates.
• Recommended Sample Dilution: 25 μL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium.
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Endocrine; Obesity; Metabolic Disease

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Conditionnement

Everything you need in a single kit.

Composants

Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Stockage et stabilité

Recommended storage for kit components is 2 - 8°C.

Autres remarques

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

Informations légales

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H332,H311,H317,H319,H411
• Conseils de prudence: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects